[HTML][HTML] Definitive hematopoietic commitment within the embryonic vascular endothelial-cadherin+ population
OBJECTIVE: The aim of this study was to assess the potential of FLK1+ and vascular
endothelial (VE)-cadherin+ populations from different stages of embryonic development to
generate hematopoietic cells ex vivo and to contribute to the hematopoietic systems of
recipient mice. MATERIALS AND METHODS: FLK1+ of VE-cadherin+ cells were isolated
from 7.5-to 9.5-dpc concepti and cultured ex vivo on OP9 stromal cells and hematopoietic
development examined. VE-cadherin+ CD45− cells from 8.5-and 9.5-dpc concepti were …
endothelial (VE)-cadherin+ populations from different stages of embryonic development to
generate hematopoietic cells ex vivo and to contribute to the hematopoietic systems of
recipient mice. MATERIALS AND METHODS: FLK1+ of VE-cadherin+ cells were isolated
from 7.5-to 9.5-dpc concepti and cultured ex vivo on OP9 stromal cells and hematopoietic
development examined. VE-cadherin+ CD45− cells from 8.5-and 9.5-dpc concepti were …