[HTML][HTML] Interaction of BiP with the J-domain of the Sec63p component of the endoplasmic reticulum protein translocation complex
B Misselwitz, O Staeck, KES Matlack… - Journal of Biological …, 1999 - ASBMB
Proteins of the Hsp70 family of ATPases interact with a conserved domain of their J-protein
partners, the J-domain, to function in numerous cellular processes. We have studied the
interaction of BiP, an Hsp70 family member in the lumen of the endoplasmic reticulum, with
the J-domain of Sec63p, a component of the Sec complex involved in post-translational
protein translocation across the endoplasmic reticulum membrane. In a real-time solid
phase binding assay, BiP binds to the immobilized Sec complex or to a fusion protein of the …
partners, the J-domain, to function in numerous cellular processes. We have studied the
interaction of BiP, an Hsp70 family member in the lumen of the endoplasmic reticulum, with
the J-domain of Sec63p, a component of the Sec complex involved in post-translational
protein translocation across the endoplasmic reticulum membrane. In a real-time solid
phase binding assay, BiP binds to the immobilized Sec complex or to a fusion protein of the …