[HTML][HTML] The methyl donor S-adenosylmethionine inhibits active demethylation of DNA: a candidate novel mechanism for the pharmacological effects of S …
N Detich, S Hamm, G Just, JD Knox, M Szyf - Journal of Biological …, 2003 - ASBMB
S-Adenosylmethionine (AdoMet) is the methyl donor of numerous methylation reactions. The
current model is that an increased concentration of AdoMet stimulates DNA
methyltransferase reactions, triggering hypermethylation and protecting the genome against
global hypomethylation, a hallmark of cancer. Using an assay of active demethylation in
HEK 293 cells, we show that AdoMet inhibits active demethylation and expression of an
ectopically methylated CMV-GFP (green fluorescent protein) plasmid in a dose-dependent …
current model is that an increased concentration of AdoMet stimulates DNA
methyltransferase reactions, triggering hypermethylation and protecting the genome against
global hypomethylation, a hallmark of cancer. Using an assay of active demethylation in
HEK 293 cells, we show that AdoMet inhibits active demethylation and expression of an
ectopically methylated CMV-GFP (green fluorescent protein) plasmid in a dose-dependent …