Characterization of CD4 and CD8 T cells producing IFN-γ in human latent and active tuberculosis

CM Rueda, ND Marín, LF García, M Rojas - Tuberculosis, 2010 - Elsevier
Patients with pulmonary tuberculosis (PTB) frequently have reduced IFN-γ production in
response to mycobacterial antigens, compared to individuals with latent Mycobacterium
tuberculosis infection (LTBi). However, it is not clear whether this reduced responsiveness is
restricted to a particular T cell subset. Herein, PBMCs from 26 PTB patients, 30 household
contacts (HHCs) of PTB, and 30 tuberculin positive (TST+) healthy subjects not recently
exposed to PTB, were stained with CFSE and stimulated non-specific (PPD) for 120 h, and …