[13] Measurement of nitric oxide-mediated effects on zinc homeostasis and zinc finger transcription factors

KD Kröncke, V Kolb-Bachofen - Methods in enzymology, 1999 - Elsevier
Publisher Summary This chapter discusses the measurement of nitric oxide (NO)-mediated
effects on zinc homeostasis and zinc finger transcription factors. The chapter describes
methods to localize and quantify intracellular free Zn 2+ in living cells after exposure to NO
and to study the effects of NO on zinc finger transcription factors in cells. NO mediates
intracytoplasmic and intranuclear Zn 2+ release that can be localized and quantified using
Zn 2+-specific fluorophores. NO-induced destruction of zinc finger domains in transcription …