[HTML][HTML] Relative and absolute quantification of postsynaptic density proteome isolated from rat forebrain and cerebellum* S
D Cheng, CC Hoogenraad, J Rush, E Ramm… - Molecular & cellular …, 2006 - ASBMB
The postsynaptic density (PSD) of central excitatory synapses is essential for postsynaptic
signaling, and its components are heterogeneous among different neuronal subtypes and
brain structures. Here we report large scale relative and absolute quantification of proteins in
PSDs purified from adult rat forebrain and cerebellum. PSD protein profiles were determined
using the cleavable ICAT strategy and LC-MS/MS. A total of 296 proteins were identified and
quantified with 43 proteins exhibiting statistically significant abundance change between …
signaling, and its components are heterogeneous among different neuronal subtypes and
brain structures. Here we report large scale relative and absolute quantification of proteins in
PSDs purified from adult rat forebrain and cerebellum. PSD protein profiles were determined
using the cleavable ICAT strategy and LC-MS/MS. A total of 296 proteins were identified and
quantified with 43 proteins exhibiting statistically significant abundance change between …