[HTML][HTML] Cross-talk between calpain and caspase proteolytic systems during neuronal apoptosis

RW Neumar, YA Xu, H Gada, RP Guttmann… - Journal of Biological …, 2003 - ASBMB
Cross-talk between calpain and caspase proteolytic systems has complicated efforts to
determine their distinct roles in apoptotic cell death. This study examined the effect of
overexpressing calpastatin, the specific endogenous calpain inhibitor, on the activity of the
two proteolytic systems following an apoptotic stimulus. Human SH-SY5Y neuroblastoma
cells were stably transfected with full-length human calpastatin cDNA resulting in 20-fold
overexpression based on Western blot and 5-fold greater calpain inhibitory activity in cell …