Determination of membrane thickness distribution from orthogonal intercepts

EB Jensen, HJG Gundersen, R Østerby - Journal of microscopy, 1979 - Wiley Online Library
EB Jensen, HJG Gundersen, R Østerby
Journal of microscopy, 1979Wiley Online Library
This report deals with the reconstruction of the distribution of membrane thickness T from
that of orthogonal intercept length L0, measured in random section planes. In such planes
the membrane appears as a band and the linear distance from one of its boundaries
perpendicular to the opposite one is the length of the orthogonal intercept. Using a
membrane model, an integral equation relating the probability density functions of
orthogonal intercept length f (l0) and membrane thickness g (τ) is derived. Relations …
Summary
This report deals with the reconstruction of the distribution of membrane thickness T from that of orthogonal intercept length L0, measured in random section planes. In such planes the membrane appears as a band and the linear distance from one of its boundaries perpendicular to the opposite one is the length of the orthogonal intercept. Using a membrane model, an integral equation relating the probability density functions of orthogonal intercept length f(l0) and membrane thickness g(τ) is derived. Relations between moments are derived and the analytic solution to the problem of reconstructing g(τ) from f(l0) is given. The parametric approach by which it is assumed that g(τ) has some known analytic form with unknown parameters is considered, and the use of a suggested analytic form for describing the thickness distribution of the human glomerular basement membrane is discussed.
Wiley Online Library