Expression of functional recombinant hemagglutinin and neuraminidase proteins from the novel H7N9 influenza virus using the baculovirus expression system
I Margine, P Palese, F Krammer - JoVE (Journal of Visualized …, 2013 - jove.com
The baculovirus expression system is a powerful tool for expression of recombinant proteins.
Here we use it to produce correctly folded and glycosylated versions of the influenza A virus
surface glycoproteins-the hemagglutinin (HA) and the neuraminidase (NA). As an example,
we chose the HA and NA proteins expressed by the novel H7N9 virus that recently emerged
in China. However the protocol can be easily adapted for HA and NA proteins expressed by
any other influenza A and B virus strains. Recombinant HA (rHA) and NA (rNA) proteins are …
Here we use it to produce correctly folded and glycosylated versions of the influenza A virus
surface glycoproteins-the hemagglutinin (HA) and the neuraminidase (NA). As an example,
we chose the HA and NA proteins expressed by the novel H7N9 virus that recently emerged
in China. However the protocol can be easily adapted for HA and NA proteins expressed by
any other influenza A and B virus strains. Recombinant HA (rHA) and NA (rNA) proteins are …