The shaping of a polyvalent and highly individual T-cell repertoire in the bone marrow of breast cancer patients

N Sommerfeldt, F Schütz, C Sohn, J Förster… - Cancer research, 2006 - AACR
N Sommerfeldt, F Schütz, C Sohn, J Förster, V Schirrmacher, P Beckhove
Cancer research, 2006AACR
We analyzed the T-cell repertoires from the bone marrow of 39 primary operated breast
cancer patients and 11 healthy female donors for the presence and frequencies of
spontaneously induced effector/memory T lymphocytes with peptide-HLA-A2-restricted
reactivity against 10 breast tumor-associated antigens (TAA) and 3 normal breast tissue–
associated antigens by short-term IFN-γ enzyme-linked immunospot (ELISpot) analysis.
Sixty-seven percent of the patients recognized TAAs with a mean frequency of 144 TAA …
Abstract
We analyzed the T-cell repertoires from the bone marrow of 39 primary operated breast cancer patients and 11 healthy female donors for the presence and frequencies of spontaneously induced effector/memory T lymphocytes with peptide-HLA-A2-restricted reactivity against 10 breast tumor-associated antigens (TAA) and 3 normal breast tissue–associated antigens by short-term IFN-γ enzyme-linked immunospot (ELISpot) analysis. Sixty-seven percent of the patients recognized TAAs with a mean frequency of 144 TAA reactive cells per 106 T cells. These patients recognized simultaneously an average of 47% of the tested TAAs. The T-cell repertoire was highly polyvalent and exhibited pronounced interindividual differences in the pattern of TAAs recognized by each patient. Strong differences of reactivity were noticed between TAAs, ranging from 100% recognition of prostate-specific antigenp141-149 to only 25% recognition of MUC1p12-20 or Her-2/neup369-377. In comparison with TAAs, reactivity to normal breast tissue–associated antigens was lower with respect to the proportions of responding patients (30%) and recognized antigens (27%), with a mean frequency of only 85/106 T cells. Healthy individuals also contained TAA-reactive T cells but this repertoire was more restricted and the frequencies were in the same range as T cells reacting to normal breast tissue–associated antigens. Our data show a highly individual T-cell repertoire for recognition of TAAs in breast cancer patients. This has potential relevance for T-cell immune diagnostics, for tumor vaccine design, and for predicting immune responsiveness. (Cancer Res 2006; 66(16): 8258-65)
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