[HTML][HTML] Novel G proteins, Rag C and Rag D, interact with GTP-binding proteins, Rag A and Rag B
T Sekiguchi, E Hirose, N Nakashima, M Ii… - Journal of Biological …, 2001 - ASBMB
Rag A/Gtr1p are G proteins and are known to be involved in the RCC1-Ran pathway. We
employed the two-hybrid method using Rag A as the bait to identify proteins binding to Rag
A, and we isolated two novel human G proteins, Rag C and Rag D. Rag C demonstrates
homology with Rag D (81.1% identity) and with Gtr2p ofSaccharomyces cerevisiae (46.1%
identity), and it belongs to the Rag A subfamily of the Ras family. Rag C and Rag D contain
conserved GTP-binding motifs (PM-1,-2, and-3) in their N-terminal regions. Recombinant …
employed the two-hybrid method using Rag A as the bait to identify proteins binding to Rag
A, and we isolated two novel human G proteins, Rag C and Rag D. Rag C demonstrates
homology with Rag D (81.1% identity) and with Gtr2p ofSaccharomyces cerevisiae (46.1%
identity), and it belongs to the Rag A subfamily of the Ras family. Rag C and Rag D contain
conserved GTP-binding motifs (PM-1,-2, and-3) in their N-terminal regions. Recombinant …