Th1 and Th17 immunocompetence in humanized NOD/SCID/IL2rγnull mice
D Rajesh, Y Zhou, E Jankowska-Gan… - Human immunology, 2010 - Elsevier
We evaluated the immunocompetence of human T cells in humanized NOD-SCID
interleukin (IL)–2r-γ-null (hu-NSG) mice bearing a human thymic organoid, after multilineage
reconstitution with isogeneic human leukocytes. Delayed type hypersensitivity (DTH)
response was assessed by a direct footpad challenge of the immunized hu-NSG host, or by
transfer of splenocytes from immunized hu-NSG, along with antigen, into footpads of CB-17
scid mice (trans vivo [tv] DTH). Both methods revealed cellular immunity to tetanus toxoid …
interleukin (IL)–2r-γ-null (hu-NSG) mice bearing a human thymic organoid, after multilineage
reconstitution with isogeneic human leukocytes. Delayed type hypersensitivity (DTH)
response was assessed by a direct footpad challenge of the immunized hu-NSG host, or by
transfer of splenocytes from immunized hu-NSG, along with antigen, into footpads of CB-17
scid mice (trans vivo [tv] DTH). Both methods revealed cellular immunity to tetanus toxoid …