Genetic engineering of murine CD8+ and CD4+ T cells for preclinical adoptive immunotherapy studies
SP Kerkar, L Sanchez-Perez, S Yang… - Journal of …, 2011 - journals.lww.com
T-cell receptor (TCR) gene therapy enables for the rapid creation of antigen-specific T cells
from mice of any strain and represents a valuable tool for preclinical immunotherapy studies.
Here, we describe the superiority of γ-retroviral vectors compared with lentiviral vectors for
transduction of murine T cells and surprisingly illustrate robust gene-transfer into
phenotypically naive/memory-stem cell like (TN/TSCM; CD62L hi/CD44 low) and central
memory (TCM; CD62L hi/CD44 hi) CD8+ T cells using murine stem cell-based γ-retroviral …
from mice of any strain and represents a valuable tool for preclinical immunotherapy studies.
Here, we describe the superiority of γ-retroviral vectors compared with lentiviral vectors for
transduction of murine T cells and surprisingly illustrate robust gene-transfer into
phenotypically naive/memory-stem cell like (TN/TSCM; CD62L hi/CD44 low) and central
memory (TCM; CD62L hi/CD44 hi) CD8+ T cells using murine stem cell-based γ-retroviral …