[HTML][HTML] Negative regulation of human growth hormone gene expression by insulin is dependent on hypoxia-inducible factor binding in primary non-tumor pituitary …
H Vakili, Y Jin, PA Cattini - Journal of Biological Chemistry, 2012 - ASBMB
Insulin controls growth hormone (GH) production at multiple levels, including via a direct
effect on pituitary somatotrophs. There are no data, however, on the regulation of the intact
human (h) GH gene (hGH1) by insulin in non-tumor pituitary cells, but the proximal promoter
region (nucleotides− 496/+ 1) responds negatively to insulin in transfected pituitary tumor
cells. A DNA-protein interaction was also induced by insulin at nucleotides− 308/− 235.
Here, we confirmed the presence of a hypoxia-inducible factor 1 (HIF-1) binding site within …
effect on pituitary somatotrophs. There are no data, however, on the regulation of the intact
human (h) GH gene (hGH1) by insulin in non-tumor pituitary cells, but the proximal promoter
region (nucleotides− 496/+ 1) responds negatively to insulin in transfected pituitary tumor
cells. A DNA-protein interaction was also induced by insulin at nucleotides− 308/− 235.
Here, we confirmed the presence of a hypoxia-inducible factor 1 (HIF-1) binding site within …