[HTML][HTML] Kinase-active interleukin-1 receptor-associated kinases promote polyubiquitination and degradation of the Pellino family: direct evidence for PELLINO …
MP Butler, JA Hanly, PN Moynagh - Journal of biological chemistry, 2007 - ASBMB
Members of the Pellino family are interleukin-1 receptor-associated kinase (IRAK)-
interacting proteins that possess RING-like domains. The presence of these domains led to
the suggestion that Pellino proteins are ubiquitin-protein isopeptide ligases (E3). However,
no conclusive data currently exist to prove this proposal. This study provides the first direct
evidence that Pellino proteins possess E3 activity. Recombinant forms of Pellino1 and
Pellino2 and both spliced variants of Pellino3 are shown in an in vitro ubiquitination assay to …
interacting proteins that possess RING-like domains. The presence of these domains led to
the suggestion that Pellino proteins are ubiquitin-protein isopeptide ligases (E3). However,
no conclusive data currently exist to prove this proposal. This study provides the first direct
evidence that Pellino proteins possess E3 activity. Recombinant forms of Pellino1 and
Pellino2 and both spliced variants of Pellino3 are shown in an in vitro ubiquitination assay to …