[HTML][HTML] Rho isoform-specific interaction with IQGAP1 promotes breast cancer cell proliferation and migration

DE Casteel, S Turner, R Schwappacher… - Journal of Biological …, 2012 - ASBMB
We performed a proteomics screen for Rho isoform-specific binding proteins to clarify the
tumor-promoting effects of RhoA and C that contrast with the tumor-suppressive effects of
RhoB. We found that the IQ-motif-containing GTPase-activating protein IQGAP1 interacts
directly with GTP-bound, prenylated RhoA and RhoC, but not with RhoB. Co-
immunoprecipitation of IQGAP1 with endogenous RhoA/C was enhanced when RhoA/C
were activated by epidermal growth factor (EGF) or transfection of a constitutively active …