Occurrence of 3 beta-hydroxy-5-cholestenoic acid, 3 beta, 7 alpha-dihydroxy-5-cholestenoic acid, and 7 alpha-hydroxy-3-oxo-4-cholestenoic acid as normal …
M Axelson, B Mörk, J Sjövall - Journal of Lipid Research, 1988 - ASBMB
Three unconjugated C27 bile acids were found in plasma from healthy humans. They were
isolated by liquid-solid extraction and anion-exchange chromatography and were identified
by gas-liquid chromatography-mass spectrometry, microchemical reactions, and ultraviolet
spectroscopy as 3 beta-hydroxy-5-cholestenoic, 3 beta, 7 alpha-dihydroxy-5-cholestenoic,
and 7 alpha-hydroxy-3-oxo-4-cholestenoic acids. Their levels often exceeded those of the
unconjugated C24 bile acids and the variations between individuals were smaller than for …
isolated by liquid-solid extraction and anion-exchange chromatography and were identified
by gas-liquid chromatography-mass spectrometry, microchemical reactions, and ultraviolet
spectroscopy as 3 beta-hydroxy-5-cholestenoic, 3 beta, 7 alpha-dihydroxy-5-cholestenoic,
and 7 alpha-hydroxy-3-oxo-4-cholestenoic acids. Their levels often exceeded those of the
unconjugated C24 bile acids and the variations between individuals were smaller than for …