An optimized two-finger archive for ZFN-mediated gene targeting
A Gupta, RG Christensen, AL Rayla, A Lakshmanan… - Nature …, 2012 - nature.com
A Gupta, RG Christensen, AL Rayla, A Lakshmanan, GD Stormo, SA Wolfe
Nature methods, 2012•nature.comThe widespread use of zinc-finger nucleases (ZFNs) for genome engineering is hampered
by the fact that only a subset of sequences can be efficiently recognized using published
finger archives. We describe a set of validated two-finger modules that complement existing
finger archives and expand the range of ZFN-accessible sequences threefold. Using this
archive, we introduced lesions at 9 of 11 target sites in the zebrafish genome.
by the fact that only a subset of sequences can be efficiently recognized using published
finger archives. We describe a set of validated two-finger modules that complement existing
finger archives and expand the range of ZFN-accessible sequences threefold. Using this
archive, we introduced lesions at 9 of 11 target sites in the zebrafish genome.
Abstract
The widespread use of zinc-finger nucleases (ZFNs) for genome engineering is hampered by the fact that only a subset of sequences can be efficiently recognized using published finger archives. We describe a set of validated two-finger modules that complement existing finger archives and expand the range of ZFN-accessible sequences threefold. Using this archive, we introduced lesions at 9 of 11 target sites in the zebrafish genome.
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