Release of the prohormone convertase PC1 from AtT-20 cells
O Vindrola, I Lindberg - Neuropeptides, 1993 - Elsevier
AtT-20 cells are known to synthesize two molecular weight forms of the prohormone
converting enzyme PC1 with molecular masses of 87 and 66 kDa. In this study we have
analyzed basal and stimulated secretion of these proteins. Western blot results show that
basal secretion medium of cultured AtT-20 cells contained low concentrations of both the 87
and 66 kDa forms of PC1 with the former protein predominant. During the stimulation period
with CRF, cAMP and cAMP+ BaCl 2, increased release of both proteins was observed, but …
converting enzyme PC1 with molecular masses of 87 and 66 kDa. In this study we have
analyzed basal and stimulated secretion of these proteins. Western blot results show that
basal secretion medium of cultured AtT-20 cells contained low concentrations of both the 87
and 66 kDa forms of PC1 with the former protein predominant. During the stimulation period
with CRF, cAMP and cAMP+ BaCl 2, increased release of both proteins was observed, but …