Introducing mothur: open-source, platform-independent, community-supported software for describing and comparing microbial communities
PD Schloss, SL Westcott, T Ryabin… - Applied and …, 2009 - Am Soc Microbiol
Applied and environmental microbiology, 2009•Am Soc Microbiol
ABSTRACT mothur aims to be a comprehensive software package that allows users to use a
single piece of software to analyze community sequence data. It builds upon previous tools
to provide a flexible and powerful software package for analyzing sequencing data. As a
case study, we used mothur to trim, screen, and align sequences; calculate distances;
assign sequences to operational taxonomic units; and describe the α and β diversity of eight
marine samples previously characterized by pyrosequencing of 16S rRNA gene fragments …
single piece of software to analyze community sequence data. It builds upon previous tools
to provide a flexible and powerful software package for analyzing sequencing data. As a
case study, we used mothur to trim, screen, and align sequences; calculate distances;
assign sequences to operational taxonomic units; and describe the α and β diversity of eight
marine samples previously characterized by pyrosequencing of 16S rRNA gene fragments …
Abstract
mothur aims to be a comprehensive software package that allows users to use a single piece of software to analyze community sequence data. It builds upon previous tools to provide a flexible and powerful software package for analyzing sequencing data. As a case study, we used mothur to trim, screen, and align sequences; calculate distances; assign sequences to operational taxonomic units; and describe the α and β diversity of eight marine samples previously characterized by pyrosequencing of 16S rRNA gene fragments. This analysis of more than 222,000 sequences was completed in less than 2 h with a laptop computer.
American Society for Microbiology