[HTML][HTML] Metabolic and genomic response to dietary isocaloric protein restriction in the rat
SC Kalhan, SO Uppal, JL Moorman, C Bennett… - Journal of biological …, 2011 - ASBMB
We have examined hepatic, genomic, and metabolic responses to dietary protein restriction
in the non-pregnant Sprague-Dawley rat. Animals were pair-fed either a 6 or 24% casein-
based diet for 7–10 days. At the end of the dietary period, a microarray analysis of the liver
was performed, followed by validation of the genes of interest. The rates of appearance of
phenylalanine, methionine, serine, and glucose and the contribution of pyruvate to serine
and glucose were quantified using tracer methods. Plasma and tissue amino acid levels …
in the non-pregnant Sprague-Dawley rat. Animals were pair-fed either a 6 or 24% casein-
based diet for 7–10 days. At the end of the dietary period, a microarray analysis of the liver
was performed, followed by validation of the genes of interest. The rates of appearance of
phenylalanine, methionine, serine, and glucose and the contribution of pyruvate to serine
and glucose were quantified using tracer methods. Plasma and tissue amino acid levels …