Lymphoid priming in human bone marrow begins before expression of CD10 with upregulation of L-selectin
LA Kohn, QL Hao, R Sasidharan, C Parekh, S Ge… - Nature …, 2012 - nature.com
Nature immunology, 2012•nature.com
Expression of the cell-surface antigen CD10 has long been used to define the lymphoid
commitment of human cells. Here we report a unique lymphoid-primed population in human
bone marrow that was generated from hematopoietic stem cells (HSCs) before onset of the
expression of CD10 and commitment to the B cell lineage. We identified this subset by high
expression of the homing molecule L-selectin (CD62L). CD10− CD62Lhi progenitors had
full lymphoid and monocytic potential but lacked erythroid potential. Gene-expression …
commitment of human cells. Here we report a unique lymphoid-primed population in human
bone marrow that was generated from hematopoietic stem cells (HSCs) before onset of the
expression of CD10 and commitment to the B cell lineage. We identified this subset by high
expression of the homing molecule L-selectin (CD62L). CD10− CD62Lhi progenitors had
full lymphoid and monocytic potential but lacked erythroid potential. Gene-expression …
Abstract
Expression of the cell-surface antigen CD10 has long been used to define the lymphoid commitment of human cells. Here we report a unique lymphoid-primed population in human bone marrow that was generated from hematopoietic stem cells (HSCs) before onset of the expression of CD10 and commitment to the B cell lineage. We identified this subset by high expression of the homing molecule L-selectin (CD62L). CD10−CD62Lhi progenitors had full lymphoid and monocytic potential but lacked erythroid potential. Gene-expression profiling placed the CD10−CD62Lhi population at an intermediate stage of differentiation between HSCs and lineage-negative (Lin−) CD34+CD10+ progenitors. CD62L was expressed on immature thymocytes, and its ligands were expressed at the cortico-medullary junction of the thymus, which suggested a possible role for this molecule in homing to the thymus. Our studies identify the earliest stage of lymphoid priming in human bone marrow.
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