[HTML][HTML] Tandem B1 elements located in a mouse methylation center provide a target for de novo DNA methylation
PA Yates, RW Burman, P Mummaneni… - Journal of Biological …, 1999 - ASBMB
A cis-acting methylation center that signalsde novo DNA methylation is located upstream of
the mouseAprt gene. In the current study, two approaches were taken to determine if tandem
B1 repetitive elements found at the 3′ end of the methylation center contribute to the
methylation signal. First, bisulfite genomic sequencing demonstrated that CpG sites within
the B1 elements were methylated at relative levels of 43% in embryonal stem cells deficient
for the maintenance DNA methyltransferase when compared with wild type embryonal stem …
the mouseAprt gene. In the current study, two approaches were taken to determine if tandem
B1 repetitive elements found at the 3′ end of the methylation center contribute to the
methylation signal. First, bisulfite genomic sequencing demonstrated that CpG sites within
the B1 elements were methylated at relative levels of 43% in embryonal stem cells deficient
for the maintenance DNA methyltransferase when compared with wild type embryonal stem …