[HTML][HTML] The type II transforming growth factor-β receptor autophosphorylates not only on serine and threonine but also on tyrosine residues
The type I and type II receptors for transforming growth factor-β (TGF-β) are structurally
related transmembrane serine/threonine kinases, which are able to physically interact with
each other at the cell surface. To help define the initial events in TGF-β signaling, we
characterized the kinase activity of the type II TGF-β receptor. A recombinant cytoplasmic
domain of the receptor was purified from Escherichia coli and baculovirus-infected insect
cells. Anti-phosphotyrosine Western blotting demonstrated that the type II receptor kinase …
related transmembrane serine/threonine kinases, which are able to physically interact with
each other at the cell surface. To help define the initial events in TGF-β signaling, we
characterized the kinase activity of the type II TGF-β receptor. A recombinant cytoplasmic
domain of the receptor was purified from Escherichia coli and baculovirus-infected insect
cells. Anti-phosphotyrosine Western blotting demonstrated that the type II receptor kinase …