[HTML][HTML] Enhancing integrin α1 inserted (I) domain affinity to ligand potentiates integrin α1β1-mediated down-regulation of collagen synthesis

M Shi, V Pedchenko, BH Greer, WD Van Horn… - Journal of Biological …, 2012 - ASBMB
Integrin α1β1 binding to collagen IV, which is mediated by the α1-inserted (I) domain, down-
regulates collagen synthesis. When unligated, a salt bridge between Arg 287 and Glu 317 is
thought to keep this domain in a low affinity conformation. Ligand binding opens the salt
bridge leading to a high-affinity conformation. How modulating integrin α1β1 affinity alters
collagen homeostasis is unknown. To address this question, we utilized a thermolysin-
derived product of the α1α2α1 network of collagen IV (α1α2α1 (IV) truncated protomer) that …