[HTML][HTML] Ubiquitination of Keap1, a BTB-Kelch substrate adaptor protein for Cul3, targets Keap1 for degradation by a proteasome-independent pathway
DD Zhang, SC Lo, Z Sun, GM Habib… - Journal of Biological …, 2005 - ASBMB
Keap1 is a BTB-Kelch protein that functions as a substrate adaptor protein for a Cul3-
dependent E3 ubiquitin ligase complex. Keap1 targets its substrate, the Nrf2 transcription
factor, for ubiquitination and subsequent degradation by the 26 S proteasome. Inhibition of
Keap1-dependent ubiquitination of Nrf2 increases steady-state levels of Nrf2 and enables
activation of cytoprotective Nrf2-dependent genes. In this report, we demonstrate that Keap1
and three other BTB-Kelch proteins, including GAN1, ENC1, and Sarcosin, are ubiquitinated …
dependent E3 ubiquitin ligase complex. Keap1 targets its substrate, the Nrf2 transcription
factor, for ubiquitination and subsequent degradation by the 26 S proteasome. Inhibition of
Keap1-dependent ubiquitination of Nrf2 increases steady-state levels of Nrf2 and enables
activation of cytoprotective Nrf2-dependent genes. In this report, we demonstrate that Keap1
and three other BTB-Kelch proteins, including GAN1, ENC1, and Sarcosin, are ubiquitinated …