Unique mechanistic insights into the beneficial effects of soluble epoxide hydrolase inhibitors in the prevention of cardiac fibrosis

P Sirish, N Li, JY Liu, KSS Lee… - Proceedings of the …, 2013 - National Acad Sciences
P Sirish, N Li, JY Liu, KSS Lee, SH Hwang, H Qiu, C Zhao, SM Ma, JE López, BD Hammock
Proceedings of the National Academy of Sciences, 2013National Acad Sciences
Tissue fibrosis represents one of the largest groups of diseases for which there are very few
effective therapies. In the heart, myocardial infarction (MI) resulting in the loss of cardiac
myocytes can culminate in adverse cardiac remodeling leading to eventual heart failure.
Adverse cardiac remodeling includes myocyte hypertrophy, fibrosis, and electrical
remodeling. We have previously demonstrated the beneficial effects of several potent
soluble epoxide hydrolase inhibitors (sEHIs) in different models of cardiac hypertrophy and …
Tissue fibrosis represents one of the largest groups of diseases for which there are very few effective therapies. In the heart, myocardial infarction (MI) resulting in the loss of cardiac myocytes can culminate in adverse cardiac remodeling leading to eventual heart failure. Adverse cardiac remodeling includes myocyte hypertrophy, fibrosis, and electrical remodeling. We have previously demonstrated the beneficial effects of several potent soluble epoxide hydrolase inhibitors (sEHIs) in different models of cardiac hypertrophy and failure. Here, we directly determine the molecular mechanisms underlying the beneficial effects of sEHIs in cardiac remodeling post-MI. Treatment with a potent sEHI, 1-trifluoromethoxyphenyl-3-(1-propionylpiperidine-4-yl)urea (TPPU), which was started 1 wk post-MI in a murine model, results in a significant improvement in cardiac function. Importantly, treatment with TPPU results in a decrease in cardiac fibrosis as quantified using histological and immunostaining techniques. Moreover, single-cell–based assays demonstrate that treatment with TPPU results in a significant decrease not only in the percentages but also the proliferative capacity of different populations of cardiac fibroblasts as well as a reduction in the migration of fibroblasts into the heart from the bone marrow. Our study provides evidence for a possible unique therapeutic strategy to reduce cardiac fibrosis and improve cardiac function post-MI.
National Acad Sciences