A semi-automated non-radioactive system for measuring recovery of RNA synthesis and unscheduled DNA synthesis using ethynyluracil derivatives
Y Nakazawa, S Yamashita, AR Lehmann, T Ogi - DNA repair, 2010 - Elsevier
Nucleotide excision repair (NER) removes the major UV-photolesions from cellular DNA. In
humans, compromised NER activity is the cause of several photosensitive diseases, one of
which is the skin-cancer predisposition disorder, xeroderma pigmentosum (XP). Two assays
commonly used in measurement of NER activity are 'unscheduled DNA synthesis (UDS)',
and 'recovery of RNA synthesis (RRS)', the latter being a specific measure of the
transcription-coupled repair sub-pathway of NER. Both assays are key techniques for …
humans, compromised NER activity is the cause of several photosensitive diseases, one of
which is the skin-cancer predisposition disorder, xeroderma pigmentosum (XP). Two assays
commonly used in measurement of NER activity are 'unscheduled DNA synthesis (UDS)',
and 'recovery of RNA synthesis (RRS)', the latter being a specific measure of the
transcription-coupled repair sub-pathway of NER. Both assays are key techniques for …