A rapid procedure for measurement of DNA repair in human fibroblasts and for complementation analysis of xeroderma pigmentosum cells
AR Lehmann, S Stevens - Mutation Research/Fundamental and Molecular …, 1980 - Elsevier
A rapid procedure for measuring unscheduled DNA synthesis has been studied in detail.
Human fibroblasts were brought into the non-dividing state by either growing to confluence
or starvation for arginine. Residual semi-conservative synthesis was abolished by
hydroxyurea. Hydroxyurea-resistant DNA synthesis which was induced by irradiation and
chemical mutagens was presumed to represent repair synthesis and provided a very rapid
semi-quantitative procedure for its measurement. Problems were encountered, however …
Human fibroblasts were brought into the non-dividing state by either growing to confluence
or starvation for arginine. Residual semi-conservative synthesis was abolished by
hydroxyurea. Hydroxyurea-resistant DNA synthesis which was induced by irradiation and
chemical mutagens was presumed to represent repair synthesis and provided a very rapid
semi-quantitative procedure for its measurement. Problems were encountered, however …