[HTML][HTML] Identification of intracellular residues in the dopamine transporter critical for regulation of transporter conformation and cocaine binding
CJ Loland, C Grånäs, JA Javitch… - Journal of Biological …, 2004 - ASBMB
Recently we showed evidence that mutation of Tyr-335 to Ala (Y335A) in the human
dopamine transporter (hDAT) alters the conformational equilibrium of the transport cycle.
Here, by substituting, one at a time, 16 different bulky or charged intracellular residues, we
identify three residues, Lys-264, Asp-345, and Asp-436, the mutation of which to alanine
produces a phenotype similar to that of Y335A. Like Y335A, the mutants (K264A, D345A,
and D436A) were characterized by low uptake capacity that was potentiated by Zn 2+ …
dopamine transporter (hDAT) alters the conformational equilibrium of the transport cycle.
Here, by substituting, one at a time, 16 different bulky or charged intracellular residues, we
identify three residues, Lys-264, Asp-345, and Asp-436, the mutation of which to alanine
produces a phenotype similar to that of Y335A. Like Y335A, the mutants (K264A, D345A,
and D436A) were characterized by low uptake capacity that was potentiated by Zn 2+ …