[HTML][HTML] Reactive oxygen species generated at mitochondrial complex III stabilize hypoxia-inducible factor-1α during hypoxia: a mechanism of O2 sensing

NS Chandel, DS McClintock, CE Feliciano… - Journal of Biological …, 2000 - ASBMB
During hypoxia, hypoxia-inducible factor-1α (HIF-1α) is required for induction of a variety of
genes including erythropoietin and vascular endothelial growth factor. Hypoxia increases
mitochondrial reactive oxygen species (ROS) generation at Complex III, which causes
accumulation of HIF-1α protein responsible for initiating expression of a luciferase reporter
construct under the control of a hypoxic response element. This response is lost in cells
depleted of mitochondrial DNA (ρ 0 cells). Overexpression of catalase abolishes hypoxic …