Cell-surface glycoproteins of human sarcomas: differential expression in normal and malignant tissues and cultured cells.

WJ Rettig, P Garin-Chesa… - Proceedings of the …, 1988 - National Acad Sciences
WJ Rettig, P Garin-Chesa, HR Beresford, HF Oettgen, MR Melamed, LJ Old
Proceedings of the National Academy of Sciences, 1988National Acad Sciences
Normal differentiation and malignant transformation of human cells are characterized by
specific changes in surface antigen phenotype. In the present study, we have defined six cell-
surface antigens of human sarcomas and normal mesenchymal cells, by using mixed
hemadsorption assays and immunochemical methods for the analysis of cultured cells and
immunohistochemical staining for the analysis of normal tissues and greater than 200 tumor
specimens. Differential patterns of F19 (Mr, 120,000/95,000 glycoprotein), F24 (Mr, 95,000 …
Normal differentiation and malignant transformation of human cells are characterized by specific changes in surface antigen phenotype. In the present study, we have defined six cell-surface antigens of human sarcomas and normal mesenchymal cells, by using mixed hemadsorption assays and immunochemical methods for the analysis of cultured cells and immunohistochemical staining for the analysis of normal tissues and greater than 200 tumor specimens. Differential patterns of F19 (Mr, 120,000/95,000 glycoprotein), F24 (Mr, 95,000 glycoprotein), G171 (Mr, 75,000 glycoprotein), G253 (Mr, 90,000 glycoprotein), S5 (Mr, 120,000 glycoprotein), and Thy-1 (Mr, 25,000 glycoprotein) antigen expression were found to characterize (i) subsets of cultured sarcoma cell lines, (ii) cultured fibroblasts derived from various organs, (iii) normal resting and activated mesenchymal tissues, and (iv) sarcoma and nonmesenchymal tumor tissues. These results provide a basic surface antigenic map for cultured mesenchymal cells and mesenchymal tissues and permit the classification of human sarcomas according to their antigenic phenotypes.
National Acad Sciences