The cAMP binding protein Epac modulates Ca2+ sparks by a Ca2+/calmodulin kinase signalling pathway in rat cardiac myocytes

L Pereira, M Métrich… - The Journal of …, 2007 - Wiley Online Library
L Pereira, M Métrich, M Fernández‐Velasco, A Lucas, J Leroy, R Perrier, E Morel…
The Journal of physiology, 2007Wiley Online Library
cAMP is a powerful second messenger whose known general effector is protein kinase A
(PKA). The identification of a cAMP binding protein, Epac, raises the question of its role in
Ca2+ signalling in cardiac myocytes. In this study, we analysed the effects of Epac activation
on Ca2+ handling by using confocal microscopy in isolated adult rat cardiomyocytes.[Ca2+] i
transients were evoked by electrical stimulation and Ca2+ sparks were measured in
quiescent myocytes. Epac was selectively activated by the cAMP analogue 8‐(4 …
cAMP is a powerful second messenger whose known general effector is protein kinase A (PKA). The identification of a cAMP binding protein, Epac, raises the question of its role in Ca2+ signalling in cardiac myocytes. In this study, we analysed the effects of Epac activation on Ca2+ handling by using confocal microscopy in isolated adult rat cardiomyocytes. [Ca2+]i transients were evoked by electrical stimulation and Ca2+ sparks were measured in quiescent myocytes. Epac was selectively activated by the cAMP analogue 8‐(4‐chlorophenylthio)‐2′‐O‐methyladenosine‐3′,5′‐cyclic monophosphate (8‐CPT). Patch‐clamp was used to record the L‐type calcium current (ICa), and Western blot to evaluate phosphorylated ryanodine receptor (RyR). [Ca2+]i transients were slightly reduced by 10 μm 8‐CPT (F/F0: decreased from 4.7 ± 0.5 to 3.8 ± 0.4, P < 0.05), an effect that was boosted when cells were previously infected with an adenovirus encoding human Epac. ICa was unaltered by Epac activation, so this cannot explain the decreased [Ca2+]i transients. Instead, a decrease in the sarcoplasmic reticulum (SR) Ca2+ load underlies the decrease in the [Ca2+]i transients. This decrease in the SR Ca2+ load was provoked by the increase in the SR Ca2+ leak induced by Epac activation. 8‐CPT significantly increased Ca2+ spark frequency (Ca2+ sparks s−1 (100 μm)−1: from 2.4 ± 0.6 to 6.9 ± 1.5, P < 0.01) while reducing their amplitude (F/F0: 1.8 ± 0.02 versus 1.6 ± 0.01, P < 0.001) in a Ca2+/calmodulin kinase II (CaMKII)‐dependent and PKA‐independent manner. Accordingly, we found that Epac increased RyR phosphorylation at the CaMKII site. Altogether, our data reveal a new signalling pathway by which cAMP governs Ca2+ release and signalling in cardiac myocytes.
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