Genetic engineering of glomerular sclerosis in the mouse via control of onset and severity of podocyte-specific injury

T Matsusaka, J Xin, S Niwa, K Kobayashi… - Journal of the …, 2005 - journals.lww.com
This study aimed to generate a mouse model of acquired glomerular sclerosis. A model
system that allows induction of podocyte injury in a manner in which onset and severity can
be controlled was designed. A transgenic mouse strain (NEP25) that expresses human
CD25 selectively in podocytes was first generated. Injection of anti-Tac (Fv)-PE38 (LMB2),
an immunotoxin with specific binding to human CD25, induced progressive nonselective
proteinuria, ascites, and edema in NEP25 mice. Podocytes showed foot process effacement …