Microdetermination of monosaccharides in glycoproteins by gas-liquid chromatography
M Tomana, W Niedermeier, C Spivey - Analytical Biochemistry, 1978 - Elsevier
M Tomana, W Niedermeier, C Spivey
Analytical Biochemistry, 1978•ElsevierIn a newly developed method for determining picomolar quantities of monosaccharides in
glycoproteins, the methyl glycosides released by methanolysis of glycoproteins are
separated as trifluoroacetates by gas-liquid chromatography and are detected with an
electron capture detector. The application of the method has been demonstrated for the
analysis of the carbohydrate moiety of secretory component isolated from human colostral
immunoglobulin A.
glycoproteins, the methyl glycosides released by methanolysis of glycoproteins are
separated as trifluoroacetates by gas-liquid chromatography and are detected with an
electron capture detector. The application of the method has been demonstrated for the
analysis of the carbohydrate moiety of secretory component isolated from human colostral
immunoglobulin A.
In a newly developed method for determining picomolar quantities of monosaccharides in glycoproteins, the methyl glycosides released by methanolysis of glycoproteins are separated as trifluoroacetates by gas-liquid chromatography and are detected with an electron capture detector. The application of the method has been demonstrated for the analysis of the carbohydrate moiety of secretory component isolated from human colostral immunoglobulin A.
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