[HTML][HTML] Clustered O-glycans of IgA1: defining macro-and microheterogeneity by use of electron capture/transfer dissociation
K Takahashi, SB Wall, H Suzuki, AD Smith… - Molecular & Cellular …, 2010 - ASBMB
IgA nephropathy (IgAN) is the most common primary glomerulonephritis in the world.
Aberrantly glycosylated IgA1, with galactose (Gal)-deficient hinge region (HR) O-glycans,
plays a pivotal role in the pathogenesis of the disease. It is not known whether the
glycosylation defect occurs randomly or preferentially at specific sites. We have described
the utility of activated ion-electron capture dissociation (AI-ECD) mass spectrometric
analysis of IgA1 O-glycosylation. However, locating and characterizing the entire range of O …
Aberrantly glycosylated IgA1, with galactose (Gal)-deficient hinge region (HR) O-glycans,
plays a pivotal role in the pathogenesis of the disease. It is not known whether the
glycosylation defect occurs randomly or preferentially at specific sites. We have described
the utility of activated ion-electron capture dissociation (AI-ECD) mass spectrometric
analysis of IgA1 O-glycosylation. However, locating and characterizing the entire range of O …