Human serum IgA1 is substituted with up to six O-glycans as shown by matrix assisted laser desorption ionisation time-of-flight mass spectrometry
E Tarelli, AC Smith, BM Hendry, SJ Challacombe… - Carbohydrate …, 2004 - Elsevier
The micro-heterogeneity of human serum IgA1 results from variable O-glycan substitutions
in the 'hinge region'of the molecule and this O-glycosylation may be altered in a number of
medical conditions. This micro-heterogeneity has been monitored by analysis of IgA1-
derived tryptic O-glycopeptides using matrix assisted laser desorption ionisation time-of-
flight mass spectrometry (MALDI-ToF-MS) analysis. With ammonium citrate–
trihydroxyacetophenone matrix, individual compositional glycoforms have been baseline …
in the 'hinge region'of the molecule and this O-glycosylation may be altered in a number of
medical conditions. This micro-heterogeneity has been monitored by analysis of IgA1-
derived tryptic O-glycopeptides using matrix assisted laser desorption ionisation time-of-
flight mass spectrometry (MALDI-ToF-MS) analysis. With ammonium citrate–
trihydroxyacetophenone matrix, individual compositional glycoforms have been baseline …