[HTML][HTML] Secretory mechanisms and intercellular transfer of microRNAs in living cells*♦
N Kosaka, H Iguchi, Y Yoshioka, F Takeshita… - Journal of Biological …, 2010 - ASBMB
The existence of circulating microRNAs (miRNAs) in the blood of cancer patients has raised
the possibility that miRNAs may serve as a novel diagnostic marker. However, the secretory
mechanism and biological function of extracellular miRNAs remain unclear. Here, we show
that miRNAs are released through a ceramide-dependent secretory machinery and that the
secretory miRNAs are transferable and functional in the recipient cells. Ceramide, whose
biosynthesis is regulated by neutral sphingomyelinase 2 (nSMase2), triggers secretion of …
the possibility that miRNAs may serve as a novel diagnostic marker. However, the secretory
mechanism and biological function of extracellular miRNAs remain unclear. Here, we show
that miRNAs are released through a ceramide-dependent secretory machinery and that the
secretory miRNAs are transferable and functional in the recipient cells. Ceramide, whose
biosynthesis is regulated by neutral sphingomyelinase 2 (nSMase2), triggers secretion of …