[HTML][HTML] Measurement of intestinal cholesterol absorption by plasma and fecal dual-isotope ratio, mass balance, and lymph fistula methods in the mouse: an analysis …
DQH Wang, MC Carey - Journal of lipid research, 2003 - ASBMB
The rate of intestinal cholesterol (Ch) absorption is an important criterion for quantitation of
Ch homeostasis. However, studies in the literature suggest that percent Ch absorption,
measured usually by a fecal dual-isotope ratio method, spans a wide range, from 20% to
90%, in healthy inbred mice on a chow diet. In the present study, we adapted four standard
methods, one direct (lymph collection) and three indirect (plasma and fecal dual-isotope
ratio, and sterol balance) measurements of Ch absorption and applied them to mice. Our …
Ch homeostasis. However, studies in the literature suggest that percent Ch absorption,
measured usually by a fecal dual-isotope ratio method, spans a wide range, from 20% to
90%, in healthy inbred mice on a chow diet. In the present study, we adapted four standard
methods, one direct (lymph collection) and three indirect (plasma and fecal dual-isotope
ratio, and sterol balance) measurements of Ch absorption and applied them to mice. Our …