Pancreatic duct epithelial cell isolation and cultivation in two‐dimensional and three‐dimensional culture systems
C Agbunag, KE Lee, S Buontempo, D Bar‐Sagi - Methods in enzymology, 2006 - Elsevier
Pancreatic ductal adenocarcinoma (PDA) is generally considered to have originated from
pancreatic duct epithelial cells (PDEC). The ability to manipulate the growth properties of
PDEC is, therefore, critical for understanding the molecular events involved in the initiation
of PDA. Here, we describe methods that we have established for the isolation and
maintenance of PDEC in two‐dimensional and three‐dimensional culture systems. The
availability of these culture systems should be particularly useful for studying their …
pancreatic duct epithelial cells (PDEC). The ability to manipulate the growth properties of
PDEC is, therefore, critical for understanding the molecular events involved in the initiation
of PDA. Here, we describe methods that we have established for the isolation and
maintenance of PDEC in two‐dimensional and three‐dimensional culture systems. The
availability of these culture systems should be particularly useful for studying their …