[HTML][HTML] Exponentially modified protein abundance index (emPAI) for estimation of absolute protein amount in proteomics by the number of sequenced peptides per …
Y Ishihama, Y Oda, T Tabata, T Sato, T Nagasu… - Molecular & Cellular …, 2005 - ASBMB
To estimate absolute protein contents in complex mixtures, we previously defined a protein
abundance index (PAI) as the number of observed peptides divided by the number of
observable peptides per protein (Rappsilber, J., Ryder, U., Lamond, AI, and Mann, M.(2002)
Large-scale proteomic analysis of the human spliceosome. Genome. Res. 12, 1231–1245).
Here we report that PAI values obtained at different concentrations of serum albumin show a
linear relationship with the logarithm of protein concentration in LC-MS/MS experiments …
abundance index (PAI) as the number of observed peptides divided by the number of
observable peptides per protein (Rappsilber, J., Ryder, U., Lamond, AI, and Mann, M.(2002)
Large-scale proteomic analysis of the human spliceosome. Genome. Res. 12, 1231–1245).
Here we report that PAI values obtained at different concentrations of serum albumin show a
linear relationship with the logarithm of protein concentration in LC-MS/MS experiments …