Imaging individual mRNA molecules using multiple singly labeled probes
Nature methods, 2008•nature.com
We describe a method for imaging individual mRNA molecules in fixed cells by probing
each mRNA species with 48 or more short, singly labeled oligonucleotide probes. This
makes each mRNA molecule visible as a computationally identifiable fluorescent spot by
fluorescence microscopy. We demonstrate simultaneous detection of three mRNA species in
single cells and mRNA detection in yeast, nematodes, fruit fly wing discs, and mammalian
cell lines and neurons.
each mRNA species with 48 or more short, singly labeled oligonucleotide probes. This
makes each mRNA molecule visible as a computationally identifiable fluorescent spot by
fluorescence microscopy. We demonstrate simultaneous detection of three mRNA species in
single cells and mRNA detection in yeast, nematodes, fruit fly wing discs, and mammalian
cell lines and neurons.
Abstract
We describe a method for imaging individual mRNA molecules in fixed cells by probing each mRNA species with 48 or more short, singly labeled oligonucleotide probes. This makes each mRNA molecule visible as a computationally identifiable fluorescent spot by fluorescence microscopy. We demonstrate simultaneous detection of three mRNA species in single cells and mRNA detection in yeast, nematodes, fruit fly wing discs, and mammalian cell lines and neurons.
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