[HTML][HTML] Phosphorylation of vimentin by Rho-associated kinase at a unique amino-terminal site that is specifically phosphorylated during cytokinesis
H Goto, H Kosako, K Tanabe, M Yanagida… - Journal of Biological …, 1998 - ASBMB
We found that vimentin, the most widely expressed intermediate filament protein, served as
an excellent substrate for Rho-associated kinase (Rho-kinase) and that vimentin
phosphorylated by Rho-kinase lost its ability to form filamentsin vitro. Two amino-terminal
sites on vimentin, Ser 38 and Ser 71, were identified as the major phosphorylation sites for
Rho-kinase, and Ser 71 was the most favored and unique phosphorylation site for Rho-
kinase in vitro. To analyze the vimentin phosphorylation by Rho-kinasein vivo, we prepared …
an excellent substrate for Rho-associated kinase (Rho-kinase) and that vimentin
phosphorylated by Rho-kinase lost its ability to form filamentsin vitro. Two amino-terminal
sites on vimentin, Ser 38 and Ser 71, were identified as the major phosphorylation sites for
Rho-kinase, and Ser 71 was the most favored and unique phosphorylation site for Rho-
kinase in vitro. To analyze the vimentin phosphorylation by Rho-kinasein vivo, we prepared …