Assay for the determination of urinary 8-hydroxy-2′-deoxyguanosine by high-performance liquid chromatography with electrochemical detection

D Germadnik, A Pilger, HW Rüdiger - Journal of Chromatography B …, 1997 - Elsevier
A high-performance liquid chromatographic procedure with electrochemical detection is
described for the determination of urinary 8-hydroxy-2′-deoxyguanosine, a major oxidative
DNA lesion induced by radical forming agents. A two-step solid-phase extraction procedure
was followed for extracting 8-hydroxy-2′-deoxyguanosine from human urine and the
analysis was performed on a RP-18 analytical column under isocratic conditions. The limit of
detection of 8-hydroxy-2′-deoxyguanosine in urine was found to be 0.9 nM. The non …