High production of SPARC/osteonectin/BM-40 in mouse metastatic B16 melanoma cell lines
Pathology oncology research, 2000•Springer
Production of SPARC/osteonectin/BM-40 was determined in mouse B16 melanoma clones
BL6 and F10 (high metastatic) and Fl (low metastatic). SPARC was produced greater
amount in BL6 and F10 than in Fl cells, showing a good agreement with their metastatic
potentials. Moreover, SPARC production was not influenced by culture pH, even in the
acidic conditions (≈ pH 5.9). Although tumor tissues show often low pH due to excessive
amount of acidic metabolites such as lactate, most studies have been done in neutral pH …
BL6 and F10 (high metastatic) and Fl (low metastatic). SPARC was produced greater
amount in BL6 and F10 than in Fl cells, showing a good agreement with their metastatic
potentials. Moreover, SPARC production was not influenced by culture pH, even in the
acidic conditions (≈ pH 5.9). Although tumor tissues show often low pH due to excessive
amount of acidic metabolites such as lactate, most studies have been done in neutral pH …
Abstract
Production of SPARC/osteonectin/BM-40 was determined in mouse B16 melanoma clones BL6 and F10 (high metastatic) and Fl (low metastatic). SPARC was produced greater amount in BL6 and F10 than in Fl cells, showing a good agreement with their metastatic potentials. Moreover, SPARC production was not influenced by culture pH, even in the acidic conditions (≈pH 5.9). Although tumor tissues show often low pH due to excessive amount of acidic metabolites such as lactate, most studies have been done in neutral pH. High SPARC production in the acidic medium, therefore, is thought to be an important potential for tumor invasive behaviour.
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