Serum-free cultures of murine adrenal cortical cells
PURPOSE: The feasibility of culturing murine adrenal cortical cells before transplantation
was investigated in this study. METHOD: Primary murine adrenal cortical cells were
maintained in either fetal bovine and horse sera–containing media or serum-free media.
Real-time polymerase chain reaction was used to quantify the levels of adrenal cortical gene
expression in the cultured cells. RESULTS: The use of sera-containing media led to the
growth of many cells in the culture, but the expression of Sf-1, Dax-1, and Cyp11b1 in such …
was investigated in this study. METHOD: Primary murine adrenal cortical cells were
maintained in either fetal bovine and horse sera–containing media or serum-free media.
Real-time polymerase chain reaction was used to quantify the levels of adrenal cortical gene
expression in the cultured cells. RESULTS: The use of sera-containing media led to the
growth of many cells in the culture, but the expression of Sf-1, Dax-1, and Cyp11b1 in such …