[HTML][HTML] Bio-samtools: Ruby bindings for SAMtools, a library for accessing BAM files containing high-throughput sequence alignments
Source code for biology and medicine, 2012•Springer
Abstract Background The SAMtools utilities comprise a very useful and widely used suite of
software for manipulating files and alignments in the SAM and BAM format, used in a wide
range of genetic analyses. The SAMtools utilities are implemented in C and provide an API
for programmatic access, to help make this functionality available to programmers wishing to
develop in the high level Ruby language we have developed bio-samtools, a Ruby binding
to the SAMtools library. Results The utility of SAMtools is encapsulated in 3 main classes …
software for manipulating files and alignments in the SAM and BAM format, used in a wide
range of genetic analyses. The SAMtools utilities are implemented in C and provide an API
for programmatic access, to help make this functionality available to programmers wishing to
develop in the high level Ruby language we have developed bio-samtools, a Ruby binding
to the SAMtools library. Results The utility of SAMtools is encapsulated in 3 main classes …
Background
The SAMtools utilities comprise a very useful and widely used suite of software for manipulating files and alignments in the SAM and BAM format, used in a wide range of genetic analyses. The SAMtools utilities are implemented in C and provide an API for programmatic access, to help make this functionality available to programmers wishing to develop in the high level Ruby language we have developed bio-samtools, a Ruby binding to the SAMtools library.
Results
The utility of SAMtools is encapsulated in 3 main classes, Bio::DB::Sam, representing the alignment files and providing access to the data in them, Bio::DB::Alignment, representing the individual read alignments inside the files and Bio::DB::Pileup, representing the summarised nucleotides of reads over a single point in the nucleotide sequence to which the reads are aligned.
Conclusions
Bio-samtools is a flexible and easy to use interface that programmers of many levels of experience can use to access information in the popular and common SAM/BAM format.
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