[HTML][HTML] The inhibitory anti-β1 integrin monoclonal antibody 13 recognizes an epitope that is attenuated by ligand occupancy: evidence for allosteric inhibition of …
AP Mould, SK Akiyama, MJ Humphries - Journal of Biological Chemistry, 1996 - ASBMB
Integrin-ligand binding causes conformational changes in the integrin, as evidenced by the
increased expression of epitopes known as ligand-induced binding sites. Some monoclonal
antibodies (mAbs) that recognize ligand-induced binding sites stimulate ligand binding,
possibly by stabilizing the ligand-occupied conformation of the integrin. Here we have
investigated the effect of ligand recognition by α5β1 on the binding of a mAb that inhibits β1
integrin function (mAb 13). Ligand (fibronectin fragment or GRGDS peptide) decreased the …
increased expression of epitopes known as ligand-induced binding sites. Some monoclonal
antibodies (mAbs) that recognize ligand-induced binding sites stimulate ligand binding,
possibly by stabilizing the ligand-occupied conformation of the integrin. Here we have
investigated the effect of ligand recognition by α5β1 on the binding of a mAb that inhibits β1
integrin function (mAb 13). Ligand (fibronectin fragment or GRGDS peptide) decreased the …