Use of a new immunoassay to measure PrPSc levels in scrapie-infected sheep brains reveals PrP genotype-specific differences
S McCutcheon, N Hunter, F Houston - Journal of immunological methods, 2005 - Elsevier
The diagnosis of prion diseases, such as scrapie and BSE, has traditionally relied upon the
identification of the disease-associated form of the prion protein, PrPSc, based on its
resistance to digestion by proteinase K (PK). A more recent development is the conformation-
dependent immunoassay (CDI), which distinguishes between PrPSc and normal PrP (PrPC)
based on their differing solubility in guanidine hydrochloride rather than resistance or
sensitivity to PK. We have developed a CDI-formatted sandwich immunoassay for the …
identification of the disease-associated form of the prion protein, PrPSc, based on its
resistance to digestion by proteinase K (PK). A more recent development is the conformation-
dependent immunoassay (CDI), which distinguishes between PrPSc and normal PrP (PrPC)
based on their differing solubility in guanidine hydrochloride rather than resistance or
sensitivity to PK. We have developed a CDI-formatted sandwich immunoassay for the …