Bilaminar co-culture of primary rat cortical neurons and glia
S Shimizu, A Abt, O Meucci - JoVE (Journal of Visualized Experiments), 2011 - jove.com
This video will guide you through the process of culturing rat cortical neurons in the
presence of a glial feeder layer, a system known as a bilaminar or co-culture model. This
system is suitable for a variety of experimental needs requiring either a glass or plastic
growth substrate and can also be used for culture of other types of neurons. Rat cortical
neurons obtained from the late embryonic stage (E17) are plated on glass coverslips or
tissue culture dishes facing a feeder layer of glia grown on dishes or plastic coverslips …
presence of a glial feeder layer, a system known as a bilaminar or co-culture model. This
system is suitable for a variety of experimental needs requiring either a glass or plastic
growth substrate and can also be used for culture of other types of neurons. Rat cortical
neurons obtained from the late embryonic stage (E17) are plated on glass coverslips or
tissue culture dishes facing a feeder layer of glia grown on dishes or plastic coverslips …